anti human mouse trem2 (R&D Systems)
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anti human mouse trem2
Anti Human Mouse Trem2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mouse+trem2/Human%2FMouse+TREM2+Alexa+Fluor%C2%AE+488-conjugated+Antibody/bio_rxiv__64898__2026__04__02__716182-287-25-29
Average 93 stars, based on 6 article reviews
Anti Human Mouse Trem2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mouse+trem2/Human%2FMouse+TREM2+Alexa+Fluor%C2%AE+488-conjugated+Antibody/bio_rxiv__64898__2026__04__02__716182-287-25-29
Average 93 stars, based on 6 article reviews
anti human mouse trem2 - by Bioz Stars,
2026-09
93/100 stars
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Incubation:Article Title: Sustained TREM2 stabilization accelerates microglia heterogeneity and Aβ pathology in a mouse model of Alzheimer’s disease Article Snippet: For measurement of cell surface TREM2 expression, BMDM were treated with either 5μm DPC333 ( ) O/N or with 50ng/ml PMA (Sigma Aldrich, 19-144) for 30 minutes. .. Cells were detached using 1X TrypLE enzyme (ThermoFisher, 12605010) and incubated with FC block (anti-CD16/32; eBioscience, 14-0161-85) in FACS buffer (PBS containing 2% FBS, 0.5mM EDTA and 0.05% Sodium azide) for 20 minutes at 4°C following which cells were stained in suspension for Article Title: Sustained Trem2 stabilization accelerates microglia heterogeneity and Aβ pathology in a mouse model of Alzheimer's disease. Article Snippet: FACS analysis of membrane TREM2 Formeasurement of cell surface Trem2 expression, BMDMwere treatedwith either 5mMDPC333 (Qian et al., 2007) O/N or with 50ng/ ml PMA (Sigma Aldrich, 19–144) for 30 min. .. Cells were detached using 1X TrypLE enzyme (ThermoFisher, 12605010) and incubated with FC block (anti-CD16/32; eBioscience, 14-0161-85) in FACS buffer (PBS containing 2% FBS, 0.5mM EDTA and 0.05% Sodium azide) for 20 min at 4 C following which cells were stained in suspension for Blocking Assay:Article Title: Sustained TREM2 stabilization accelerates microglia heterogeneity and Aβ pathology in a mouse model of Alzheimer’s disease Article Snippet: For measurement of cell surface TREM2 expression, BMDM were treated with either 5μm DPC333 ( ) O/N or with 50ng/ml PMA (Sigma Aldrich, 19-144) for 30 minutes. .. Cells were detached using 1X TrypLE enzyme (ThermoFisher, 12605010) and incubated with FC block (anti-CD16/32; eBioscience, 14-0161-85) in FACS buffer (PBS containing 2% FBS, 0.5mM EDTA and 0.05% Sodium azide) for 20 minutes at 4°C following which cells were stained in suspension for Article Title: Sustained Trem2 stabilization accelerates microglia heterogeneity and Aβ pathology in a mouse model of Alzheimer's disease. Article Snippet: FACS analysis of membrane TREM2 Formeasurement of cell surface Trem2 expression, BMDMwere treatedwith either 5mMDPC333 (Qian et al., 2007) O/N or with 50ng/ ml PMA (Sigma Aldrich, 19–144) for 30 min. .. Cells were detached using 1X TrypLE enzyme (ThermoFisher, 12605010) and incubated with FC block (anti-CD16/32; eBioscience, 14-0161-85) in FACS buffer (PBS containing 2% FBS, 0.5mM EDTA and 0.05% Sodium azide) for 20 min at 4 C following which cells were stained in suspension for FACS:Article Title: Sustained TREM2 stabilization accelerates microglia heterogeneity and Aβ pathology in a mouse model of Alzheimer’s disease Article Snippet: For measurement of cell surface TREM2 expression, BMDM were treated with either 5μm DPC333 ( ) O/N or with 50ng/ml PMA (Sigma Aldrich, 19-144) for 30 minutes. .. Cells were detached using 1X TrypLE enzyme (ThermoFisher, 12605010) and incubated with FC block (anti-CD16/32; eBioscience, 14-0161-85) in FACS buffer (PBS containing 2% FBS, 0.5mM EDTA and 0.05% Sodium azide) for 20 minutes at 4°C following which cells were stained in suspension for Article Title: Sustained Trem2 stabilization accelerates microglia heterogeneity and Aβ pathology in a mouse model of Alzheimer's disease. Article Snippet: FACS analysis of membrane TREM2 Formeasurement of cell surface Trem2 expression, BMDMwere treatedwith either 5mMDPC333 (Qian et al., 2007) O/N or with 50ng/ ml PMA (Sigma Aldrich, 19–144) for 30 min. .. Cells were detached using 1X TrypLE enzyme (ThermoFisher, 12605010) and incubated with FC block (anti-CD16/32; eBioscience, 14-0161-85) in FACS buffer (PBS containing 2% FBS, 0.5mM EDTA and 0.05% Sodium azide) for 20 min at 4 C following which cells were stained in suspension for Staining:Article Title: Sustained TREM2 stabilization accelerates microglia heterogeneity and Aβ pathology in a mouse model of Alzheimer’s disease Article Snippet: For measurement of cell surface TREM2 expression, BMDM were treated with either 5μm DPC333 ( ) O/N or with 50ng/ml PMA (Sigma Aldrich, 19-144) for 30 minutes. .. Cells were detached using 1X TrypLE enzyme (ThermoFisher, 12605010) and incubated with FC block (anti-CD16/32; eBioscience, 14-0161-85) in FACS buffer (PBS containing 2% FBS, 0.5mM EDTA and 0.05% Sodium azide) for 20 minutes at 4°C following which cells were stained in suspension for Article Title: Sustained Trem2 stabilization accelerates microglia heterogeneity and Aβ pathology in a mouse model of Alzheimer's disease. Article Snippet: FACS analysis of membrane TREM2 Formeasurement of cell surface Trem2 expression, BMDMwere treatedwith either 5mMDPC333 (Qian et al., 2007) O/N or with 50ng/ ml PMA (Sigma Aldrich, 19–144) for 30 min. .. Cells were detached using 1X TrypLE enzyme (ThermoFisher, 12605010) and incubated with FC block (anti-CD16/32; eBioscience, 14-0161-85) in FACS buffer (PBS containing 2% FBS, 0.5mM EDTA and 0.05% Sodium azide) for 20 min at 4 C following which cells were stained in suspension for Suspension:Article Title: Sustained TREM2 stabilization accelerates microglia heterogeneity and Aβ pathology in a mouse model of Alzheimer’s disease Article Snippet: For measurement of cell surface TREM2 expression, BMDM were treated with either 5μm DPC333 ( ) O/N or with 50ng/ml PMA (Sigma Aldrich, 19-144) for 30 minutes. .. Cells were detached using 1X TrypLE enzyme (ThermoFisher, 12605010) and incubated with FC block (anti-CD16/32; eBioscience, 14-0161-85) in FACS buffer (PBS containing 2% FBS, 0.5mM EDTA and 0.05% Sodium azide) for 20 minutes at 4°C following which cells were stained in suspension for Article Title: Sustained Trem2 stabilization accelerates microglia heterogeneity and Aβ pathology in a mouse model of Alzheimer's disease. Article Snippet: FACS analysis of membrane TREM2 Formeasurement of cell surface Trem2 expression, BMDMwere treatedwith either 5mMDPC333 (Qian et al., 2007) O/N or with 50ng/ ml PMA (Sigma Aldrich, 19–144) for 30 min. .. Cells were detached using 1X TrypLE enzyme (ThermoFisher, 12605010) and incubated with FC block (anti-CD16/32; eBioscience, 14-0161-85) in FACS buffer (PBS containing 2% FBS, 0.5mM EDTA and 0.05% Sodium azide) for 20 min at 4 C following which cells were stained in suspension for Bicinchoninic Acid Protein Assay:Article Title: Opposite microglial activation stages upon loss of PGRN or TREM 2 result in reduced cerebral glucose metabolism Article Snippet: Approximately 10–20 mg of powdered mouse brain from Grn −/− and wt mice (3–4, 6, 13, 16–18 months of age, female and male mice as indicated in the source data file to fig 2) was homogenized in Tris‐buffered saline (TBS) supplemented with protease inhibitor cocktail (Sigma‐Aldrich) and centrifuged for 30 min, 15,000 × g , 4°C. .. Protein concentrations of supernatants were determined using the BCA protein assay (Pierce, Thermo Scientific), and equal amount of protein was analyzed for TREM2. sTREM2 levels in mouse serum or mouse brain extracts were quantified using the Mesoscale platform essentially as described previously (Kleinberger et al , ) using 0.25 μg/ml biotinylated polyclonal goat Immunoprecipitation:Article Title: The FTD ‐like syndrome causing TREM 2 T66M mutation impairs microglia function, brain perfusion, and glucose metabolism Article Snippet: Images for immunoblot quantifications were obtained on a Fusion SL imager (PeqLab) and analyzed using Multi Gauge V3.0 (PLX Devices). .. For immunoprecipitation from total mouse brain lysates, biotinylated goat Article Title: The FTD ‐like syndrome causing TREM 2 T66M mutation impairs microglia function, brain perfusion, and glucose metabolism Article Snippet: Images for immunoblot quantifications were obtained on a Fusion SL imager (PeqLab) and analyzed using Multi Gauge V3.0 (PLX Devices). .. Antibodies For immunoprecipitation from total mouse brain lysates, biotinylated goat |
